Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: High-parameter immune profiling with CyTOF

doi: 10.1007/978-1-4939-9773-2_16

Figure Lengend Snippet: Reference Panel (Hartmann et al; Submitted)

Article Snippet: CD8 CAR-T cells were further divided into subsets showing the presence of T stem cell memory (Tscm) and T central memory (Tcm) cells. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Mass Element Marker Biology Staining Source dilution catalogue 89 Y CD45 Pan Immune surface Fluidigm 100 3089003B 139 La CD235ab/CD61 Dump BioLegend 100 custom 140 Ce 141 Pr CD3 Pan T Cells surface BioLegend 100 custom 142 Nd CD19 Pan B Cells surface Fluidigm 100 3142001B 143 Nd CD117 Mast, early immune surface Fluidigm 100 3143001B 144 Nd CD11b Mac / Mono surface Fluidigm 400 3144001B 145 Nd CD4 Tcell Sub / Mono surface Fluidigm 200 3145001B 146 Nd CD8a Tcell Sub / NK surface Fluidigm 1600 3146001B 147 Sm CD11c DC / Mac / Mono surface Fluidigm 200 3147008B 148 Nd CD14 Mono/Mac surface Fluidigm 1600 3148010B 149 Sm 150 Nd FceRI IgE Receptor (Mast/Basophil) surface Fluidigm 100 3150027B 151 Eu CD123 Bcell Sub, DC, Baso, pDC surface Fluidigm 100 3151001B 152 Sm gdTCR gd T Cells surface Fluidigm 100 3152008B 153 Eu CD45RA T Cell Naïve/Mem surface Fluidigm 100 3153001B 154 Sm TIM3 Th1 polarization surface Fluidigm 100 3154010B 155 Gd 156 Gd PD-L1 (CD274) Checkpoint surface Fluidigm 100 3156026B 157 Gd 158 Gd CD27 B/T Cell Mem surface Fluidigm 400 3158010B 159 Tb 160 Gd Tbet Th1 polarization/NK intracellular Fluidigm 100 3160010B 161 Dy CD152 (CTLA-4) Checkpoint intracellular Fluidigm 800 3161004B 162 Dy FoxP3 Treg intracellular Fluidigm 400 3162011A 163 Dy CD33 Pan Myeloid surface Fluidigm 100 3163023B 164 Dy CD45RO T Cell Naïve/Mem surface Fluidigm 200 3164007B 165 Ho CD127 T cell Sub, Treg surface Fluidigm 100 3165008B 166 Er 167 Er CCR7 (CD197) T Cell subset (eff/mem) surface Fluidigm 100 3167009A 168 Er Ki-67 Proliferation intracellular Fluidigm 400 3168007B 169 Tm CD25 Treg surface Fluidigm 100 3169003B 170 Er TCR Va24-Ja18 iNKT intracellular Fluidigm 100 3170015B 171 Yb 172 Yb CD38 B Cell, NK, Plasma surface Fluidigm 400 3172007B 173 Yb 174 Yb HLA-DR APC surface Fluidigm 100 3174001B 175 Lu PD-1 Checkpoint surface Fluidigm 100 3175008B 176 Yb CD56 NK surface Fluidigm 100 3176008B 209 Bi CD16 Fc Receptor, NK, Neu, Mono surface Fluidigm 100 3209002B Open in a separate window Reference Panel (Hartmann et al; Submitted) The advantages of adopting mass cytometry by time of flight (CyTOF) methods as opposed to high dimensional flow cytometry include( Bendall et al. 2012 ): list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Negligible signal overlap between parameters, creating a lower background and little or no need for signal compensation.

Techniques: Marker, Staining, Clinical Proteomics